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| Rocha,Carlos Alberto Machado da; Cunha,Lorena Araújo da; Pinheiro,Raul Henrique da Silva; Bahia,Marcelo de Oliveira; Burbano,Rommel Mario Rodríguez. |
| The frequencies of micronuclei (MN) and morphological nuclear abnormalities (NA) in erythrocytes in the peripheral blood of tambaqui (Colossoma macropomum), treated with 2 mg.L-1 methylmercury (MeHg), were analyzed. Two groups (nine specimens in each) were exposed to MeHg for different periods (group A - 24 h; group B - 120 h). A third group served as negative control (group C, untreated; n = 9). Although, when compared to the control group there were no significant differences in MN frequency in the treated groups, for NA, the differences between the frequencies of group B (treated for 120 h) and the control group were extremely significant (p < 0.02), thus demonstrating the potentially adverse effects of MeHg on C. macropomum erythrocytes after... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Genotoxicity; Methylmercury; Micronuclei assay. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400024 |
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| MONTE,ZENAIDE S.; SILVA,AMANDA M.; LIMA,GLÁUCIA M.S.; SILVA,TERESINHA G. DA; MARQUES,KARLA M.R.; RODRIGUES,MARIA D.; FALCÃO,EMERSON P.S.; MELO,SEBASTIÃO J.. |
| ABSTRACT A series of arylamidines 3a-j was designed, synthesized and investigated for antimicrobial activity. Structures of the compounds were confirmed by IR, 1H-NMR and 13C-NMR and a 2D spectroscopic study was performed. A preliminary screening of the antimicrobial tests clearly showed that three out of ten arylamidines, viz, 3f, 3g and 3i, were effective against all the gram-negative bacteria: Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella enteric; and against the yeast, candida albicans. Further, the Minimum Inhibitory Concentrations (MIC) against the bacteria and yeast were determined. All compounds 3a-d, 3f, 3g, 3i and 3j were also investigated for their low cytotoxic effects on tested cell lines. Compounds 3d and 3f were the most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arylamidines; 2D spectroscopy; Gram-negative bacteria; Yeast; Cytotoxicity. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301051 |
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| Sai,Ma; Le-qun,Shan; Yu-hong,Xiao; Fang,Li; Li,Huang; Shen,Lijuan; Ji-hua,Chen. |
| Antibacterial monomers incorporated in dentin bonding systems may have toxic effects on the pulp. Thus, the cytotoxicity of antibacterial monomers and its underlying mechanisms must be elucidated to improve the safety of antibacterial monomer application. The influence of an antibacterial monomer, methacryloxylethyl cetyl ammonium chloride (DMAE-CB), on the vitality of L929 mouse fibroblasts was tested using MTT assay. Cell cycle progression was studied using flow cytometry. Production of intracellular reactive oxygen species (ROS) after DMAE-CB treatment was measured using 2,7-dichlorodihydrofluorescein diacetate staining and flow cytometry analysis. Loss of mitochondrial membrane potential, disturbance of Bcl-2 and Bax expression, as well as release of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quaternary ammonium compounds; Dental monomer; Cytotoxicity; Apoptosis; Reactive oxygen species; Methacryloxylethyl cetyl ammonium chloride. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100008 |
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| Demir,Leyla; Toğar,Başak; Türkez,Hasan; Sozio,Piera; Aslan,Ali; Stefano,Antonio Di. |
| Diffractaic acid (DA) is a naturally occurring depside derivative found in several lichen species. It has a wide range of important biological effects such as analgesic and antiviral properties, although its cytotoxic, cytogenetic and oxidative effects have not been investigated in human blood tissue yet. Therefore, increasing concentrations (1, 5, 10, 25, 50, 100 and 200 mgL-1) of DA was added into human whole blood cultures. 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyl tetrazolium bromide (MTT) assay was used to assess the cell viability and/or cytotoxicity and genotoxic damage potential of DA using chromosome aberration (CA) and micronucleus (MN) tests were performed. In addition, oxidative alterations were determined by the total antioxidant capacity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diffractaic acid; Cytotoxicity; Human blood; Genotoxicity; Oxidative status. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100075 |
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| Turkez,Hasan; Sonmez,Erdal; Turkez,Ozlem; Mokhtar,Yousef Ibrahem; Stefano,Antonio Di; Turgut,Guven. |
| Tungsten (VI) oxide (WO3) nanoparticles (NPs) are used for many industrial purposes in everyday life. However, their effects on human health have not been sufficiently evaluated. Therefore, the present study was designed to investigate the toxicity potentials of various concentrations (0 to 1000 ppm) of WO3NPs (<100 nm particle size) in cultured primary rat hepatocytes. The results of cell viability assay showed that the higher concentrations of dispersed WO3 NPs (300, 500 and 1000 ppm) caused significant (p<0.05) decreases of cell viability. Also, dose dependent negative alterations were observed in oxidative status and antioxidant capacity levels after the application of WO3 in cultured rat primary hepatocytes. The results of genotoxicity tests... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tungsten oxide; Nanotoxicity; Genotoxicity; Cytotoxicity; Hepatocytes; Oxidative stress. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400532 |
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| Sales, Ila Monize Sousa; Oliveira, Jussara Damascena de; Santos, Fabelina Karollyne Silva dos; Feitoza, Lidiane de Lima; Sousa, João Marcelo de Castro e; Peron, Ana Paula. |
| The goal of the present study was to evaluate the cytotoxicity and genotoxicity of artificial synthetic flavoring agents Cookie and Tutti-frutti. To this end, root meristem cells of Allium cepa L. were exposed to these substances in exposure times of 24 and 48 hours using individual doses of 0.3; 0.6 and 0.9 mL and doses combined as follows: 0.3 mL + 0.3 mL; 0.6 mL and 0.9 mL + 0.6 mL + 0.9 mL. After applying the treatments, root meristems were fixed, hydrolyzed, stained and analyzed a total of 5,000 cells using an optical microscope to evaluate each dose and combined treatment. All three doses of Cookie flavoring and combined treatments significantly inhibited cell division of the tissue studied. Doses of Tutti-Frutti caused no change in cell division... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity flavorings; Cell division; Cell aberrations; Cytotoxicity; Genotoxicity. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30751 |
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| Silva, Sara Iolanda Oliveira da; Santana, Gleuvânia Marques; Sales, Ila Monize Sousa; Sousa, João Marcelo de Castro e; Peron, Ana Paula. |
| This study aimed to examine the cytotoxicity and genotoxicity of synthetic flavorings, nature identical, Chocolate, Strawberry and Condensed Milk. This evaluation was performed in root meristem cells of Allium cepa L., in exposure times of 24 and 48 hours and using doses of 0.2; 0.4 and 0.6 mL, in combination, in which one of the three doses of a flavoring was combined with a different dose of one of the two other flavor additives studied. Roots were fixed in Carnoy’s solution, hydrolyzed in hydrochloric acid, stained with acetic orcein and then analyzed, under light microscopy, 5,000 cells for each treatment. For data analysis, it was used Chi-square test at 5%. All the treatments with combinations between the flavorings Chocolate/Strawberry and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aroma and flavor additives; Cytotoxicity; Genotoxicity A. cepa Citogenética. |
| Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/30475 |
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| Avelar-Freitas,B.A.; Almeida,V.G.; Pinto,M.C.X.; Mourão,F.A.G.; Massensini,A.R.; Martins-Filho,O.A.; Rocha-Vieira,E.; Brito-Melo,G.E.A.. |
| Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the expertise of the analyst, can affect the results. We propose an alternative assay for evaluating cell viability that combines the TB exclusion test and the high sensitivity of the flow cytometry technique. Previous studies have demonstrated the ability of TB to emit fluorescence when complexed with proteins. According to our results, TB/bovine serum albumin and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypan blue; Fluorescence; Flow cytometry; Cytotoxicity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400307 |
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| Sun,Yi Fu; Shao,Li Wei; Chen,Qi; Gao,Xu; Li,Fang; Wu,Chang Yan. |
| Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The cell viability curves showed that the two compounds have anti-proliferation activity on spinal tumor cells, and the activity of compound 1 is higher than compound 2. The annexin V-FITC/PI assay and western... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coordination polymers; Glioma cell strains; Cytotoxicity; Apoptosis; Immunoblotting. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000500608 |
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| BASTOS,ISLA V.G.A.; OLIVEIRA,TATIANE B. DE; RODRIGUES,MARIA D.; MILITÃO,GARDÊNIA C.G.; SILVA,TERESINHA G. DA; TURATTI,IZABEL C.C.; LOPES,NORBERTO P.; MELO,SEBASTIÃO J. DE. |
| ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Gas Chromatography; Phoradendron microphyllum; Phoradendron mucronatum. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991 |
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| Illanchezian,Seethalakshmi; Jayaraman,SathishKumar; Manoharan,Muthu Saravanan; Valsalam,Saritha. |
| The present study was conducted to determine the virulence and cytotoxicity of Aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in Chennai, Tamil Nadu, India. Among 73 A. hydrophila strains isolated from fish and shrimp samples, 86.3% exhibited haemolysis, 78.1% produced slime, 98.63% produced protease and also demonstrated cytotoxicity on Vero cells. Cell shrinkage, detachment and rounding of Vero cells were recorded as cytotoxic changes. Only one strain did not show haemolysis, slime production, proteolytic activity and cytotoxicity on treatment with Vero cells. Positive correlation was observed between proteolytic activity and cytotoxicity irrespective of haemolytic activity of the strains. These results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aeromonas hydrophila; Seafood; Virulence; Cytotoxicity; Vero cells. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400016 |
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| Registros recuperados: 116 | |
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